CD69 Antibody (D-3): sc-373799

CD69 Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD69 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CD69 (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD69, also known as the activation inducer molecule (AIM), is expressed as a disulfide-linked homodimer and plays a crucial role in the immune response by acting as one of the earliest markers of T cell, B cell, and natural killer cell activation. CD69 presence on activated lymphocytes is essential for the regulation of immune responses, as CD69 influences cell proliferation, cytokine production, and the overall activation state of immune cells. Notably, CD69 is constitutively expressed on platelets, CD4+ or CD8+ thymocytes, and germinal center T cells, while being absent from resting lymphocytes, highlighting CD69 importance as a marker for cellular activation and potential role in various immunological disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD69 Antibody (D-3) References:

1. Crystal structure of human CD69: a C-type lectin-like activation marker of hematopoietic cells.  |  Natarajan, K., et al. 2000. Biochemistry. 39: 14779-86. PMID: 11101293
2. CD69 Deficiency Enhances the Host Response to Vaccinia Virus Infection through Altered NK Cell Homeostasis.  |  Notario, L., et al. 2016. J Virol. 90: 6464-6474. PMID: 27147744
3. Role of CD69 in the pathogenesis of elastase-induced pulmonary inflammation and emphysema.  |  Fujita, T., et al. 2016. Biochem Biophys Rep. 7: 400-407. PMID: 28955931
4. Implication of CD69+ CD103+ tissue-resident-like CD8+ T cells as a potential immunotherapeutic target for cholangiocarcinoma.  |  Kim, HD., et al. 2021. Liver Int. 41: 764-776. PMID: 33548061
5. Activin A downregulates the CD69-MT2A axis via p38MAPK to induce erythroid differentiation that sensitizes BCR-ABL-positive cells to imatinib.  |  Chen, CW., et al. 2022. Exp Cell Res. 417: 113219. PMID: 35643179
6. CD69 and SBK1 as potential predictors of responses to PD-1/PD-L1 blockade cancer immunotherapy in lung cancer and melanoma.  |  Hu, ZW., et al. 2022. Front Immunol. 13: 952059. PMID: 36045683
7. The Expression of Activation Markers CD25 and CD69 Increases during Biologic Treatment of Psoriasis.  |  Adamczyk, M., et al. 2023. J Clin Med. 12: PMID: 37892710
8. The CD69 receptor: a multipurpose cell-surface trigger for hematopoietic cells.  |  Testi, R., et al. 1994. Immunol Today. 15: 479-83. PMID: 7945773
9. Molecular characterization of the early activation antigen CD69: a type II membrane glycoprotein related to a family of natural killer cell activation antigens.  |  Ziegler, SF., et al. 1993. Eur J Immunol. 23: 1643-8. PMID: 8100776
10. Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors.  |  López-Cabrera, M., et al. 1993. J Exp Med. 178: 537-47. PMID: 8340758
11. Expression cloning of the early activation antigen CD69, a type II integral membrane protein with a C-type lectin domain.  |  Hamann, J., et al. 1993. J Immunol. 150: 4920-7. PMID: 8496594
12. Multiple dimeric forms of human CD69 result from differential addition of N-glycans to typical (Asn-X-Ser/Thr) and atypical (Asn-X-cys) glycosylation motifs.  |  Vance, BA., et al. 1997. J Biol Chem. 272: 23117-22. PMID: 9287313

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD69 Antibody (D-3) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-CD69 Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD69 Antibody (D-3) Specifications

Alternate Product Name: CD69

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-199

Epitope Target: CD69

Epitope Species: human

Product Reactivity/Detection: CD69

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for CD69 Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin