CD161 Double Nickase Plasmid (h): sc-405895-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD161 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD161 Double Nickase Plasmid (h) and CD161 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KLRB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD161 Antibody (B199.2): sc-58963
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD161 Double Nickase Plasmid (h) | sc-405895-NIC | 20 µg | $410.00 | |||
CD161 Double Nickase Plasmid (h2) | sc-405895-NIC-2 | 20 µg | $410.00 |
KLRB1 encodes CD161, a C-type lectin-like receptor prominently expressed on subsets of natural killer cells and T cells, where it helps tune activation thresholds, cytokine secretion, and cytotoxic effector functions. CD161 participates in immunoregulatory circuits governing lymphocyte differentiation and tissue homing, influencing inflammatory signaling programs and immune surveillance. Altered KLRB1/CD161 expression and CD161+ lymphocyte frequencies have been associated with immune dysregulation in chronic inflammation, autoimmunity, and tumor immune microenvironments. As a cell-surface marker linked to functional lymphocyte states, CD161 is frequently leveraged in flow cytometry-based immunophenotyping and mechanistic studies of NK/T-cell responses.
CD161 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KLRB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KLRB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KLRB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KLRB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.