CBX8 Double Nickase Plasmid (h): sc-407903-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CBX8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CBX8 Double Nickase Plasmid (h) and CBX8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CBX8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CBX8 Antibody (C-3): sc-374332
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CBX8 Double Nickase Plasmid (h) | sc-407903-NIC | 20 µg | $410.00 | |||
CBX8 Double Nickase Plasmid (h2) | sc-407903-NIC-2 | 20 µg | $410.00 |
CBX8 (chromobox 8) is a Polycomb group protein that functions as a chromatin reader within canonical Polycomb repressive complex 1 (PRC1), recognizing repressive histone marks and helping maintain transcriptional silencing programs. Through PRC1-mediated chromatin compaction and regulation of histone ubiquitination, CBX8 contributes to stable control of developmental genes, cell identity, and cell-cycle–linked transcriptional states. Dysregulated CBX8 activity has been associated with altered epigenetic landscapes that influence proliferation, differentiation, and senescence-related pathways in multiple disease contexts. Its role in transcriptional repression makes CBX8 a useful target for investigating how Polycomb-dependent chromatin regulation shapes gene networks.
CBX8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CBX8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CBX8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CBX8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CBX8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.