CaMKKβ Antibody (C-11): sc-271674

CaMKKβ Antibody (C-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects CaMKKβ protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CaMKKβ antibody (C-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. CaMKKβ plays a crucial role in cellular signaling pathways by acting as a key regulator of calcium/calmodulin-dependent protein kinases, specifically CaMKI and CaMKIV, which are essential for various physiological processes, including muscle contraction, neurotransmitter release, and gene expression. Activation of these kinases depends on phosphorylation of specific threonine residues, with CaMKKβ phosphorylating the regulatory threonine at position 177 of CaMKI and threonine 200 of CaMKIV, thereby facilitating their full activation. This regulatory mechanism is vital for maintaining cellular homeostasis and responding to changes in calcium levels, making CaMKKβ an important target for research in various fields, including neurobiology and cardiovascular studies.

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CaMKKβ Antibody (C-11) References:

1. Characterization of the CaMKKβ-AMPK signaling complex.  |  Green, MF., et al. 2011. Cell Signal. 23: 2005-12. PMID: 21807092
2. Circ_0000518 Promotes Macrophage/Microglia M1 Polarization via the FUS/CaMKKβ/AMPK Pathway to Aggravate Multiple Sclerosis.  |  Jiang, F., et al. 2022. Neuroscience. 490: 131-143. PMID: 34920022
3. Quercetin induces autophagy-associated death in HL-60 cells through CaMKKβ/AMPK/mTOR signal pathway.  |  Xiao, J., et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54: 1244-1256. PMID: 36148953
4. Bufalin induces apoptosis and autophagy via the Ca2+/CaMKKβ/AMPK/Beclin1 signaling pathway in osteosarcoma cells.  |  Zheng, YD., et al. 2023. Cell Biol Int. 47: 1344-1353. PMID: 36987947
5. Transcriptional, biochemical, and immunohistochemical analyses of CaMKKβ/2 splice variants that co-localize with CaMKIV in spermatids.  |  Ohtsuka, S., et al. 2024. Cell Calcium. 117: 102820. PMID: 37979343
6. Human calcium-calmodulin dependent protein kinase I: cDNA cloning, domain structure and activation by phosphorylation at threonine-177 by calcium-calmodulin dependent protein kinase I kinase.  |  Haribabu, B., et al. 1995. EMBO J. 14: 3679-86. PMID: 7641687
7. Characterization of a Ca2+/calmodulin-dependent protein kinase cascade. Molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase.  |  Tokumitsu, H., et al. 1995. J Biol Chem. 270: 19320-4. PMID: 7642608
8. Calcium/calmodulin-dependent protein kinase II downregulates both calcineurin and protein kinase C-mediated pathways for cytokine gene transcription in human T cells.  |  Hama, N., et al. 1995. J Exp Med. 181: 1217-22. PMID: 7869038
9. cDNA cloning and expression of human calmodulin-dependent protein kinase IV.  |  Kitani, T., et al. 1994. J Biochem. 115: 637-40. PMID: 8089075
10. G1 cell cycle arrest and apoptosis are induced in NIH 3T3 cells by KN-93, an inhibitor of CaMK-II (the multifunctional Ca2+/CaM kinase).  |  Tombes, RM., et al. 1995. Cell Growth Differ. 6: 1063-70. PMID: 8519682
11. Activation of Ca2+/calmodulin-dependent protein kinase (CaM-kinase) IV by CaM-kinase kinase in Jurkat T lymphocytes.  |  Park, IK. and Soderling, TR. 1995. J Biol Chem. 270: 30464-9. PMID: 8530476
12. Components of a calmodulin-dependent protein kinase cascade. Molecular cloning, functional characterization and cellular localization of Ca2+/calmodulin-dependent protein kinase kinase beta.  |  Anderson, KA., et al. 1998. J Biol Chem. 273: 31880-9. PMID: 9822657

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CaMKKβ Antibody (C-11) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-CaMKKβ Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CaMKKβ Antibody (C-11) Specifications

Alternate Product Name: calcium/calmodulin-dependent (CaM) kinase kinase (CAMKK2)

Product Clone Name: C-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-95

Epitope Target: CaMKKβ

Epitope Species: human

Product Reactivity/Detection: CaMKKβ

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for CaMKKβ Antibody (C-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin