Calcyclin Antibody (7D11A8): sc-53950

Calcyclin Antibody (7D11A8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Calcyclin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Calcyclin antibody (7D11A8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Calcyclin, also known as Prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9, S-100 calcium-binding protein A6 (S-100A6), or MLN 4, is a homodimeric member of the S-100 calcium-binding protein family, which plays a crucial role in calcium signal transduction. Calcyclin regulates various cellular processes, including cell growth, proliferation, secretion, and exocytosis, making Calcyclin a key player in cellular responses to growth factors. Calcyclin expression is notably upregulated in proliferating and differentiating cells, and Calcyclin is often overexpressed in acute myeloid leukemias. Cell-specific expression is observed in subpopulations of neurons, astrocytes, epithelial cells, and fibroblasts, highlighting Calcyclin′s diverse functional roles across different cell types. Calcyclin serves as a specific target for the S-100B protein in vivo, with binding stabilized by calcium and zinc, further emphasizing Calcyclin′s significance in cellular signaling pathways. Calcyclin also interacts with Annexin XI and CacyBP (calcyclin-binding protein), underscoring Calcyclin′s involvement in complex protein networks that regulate critical cellular functions.

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Calcyclin Antibody (7D11A8) References:

1. Increased expression of S100A6 (Calcyclin), a calcium-binding protein of the S100 family, in human colorectal adenocarcinomas.  |  Komatsu, K., et al. 2000. Clin Cancer Res. 6: 172-7. PMID: 10656447
2. Characterization of the interaction of calcyclin (S100A6) and calcyclin-binding protein.  |  Nowotny, M., et al. 2000. J Biol Chem. 275: 31178-82. PMID: 10884380
3. S100A6 and S100A11 are specific targets of the calcium- and zinc-binding S100B protein in vivo.  |  Deloulme, JC., et al. 2000. J Biol Chem. 275: 35302-10. PMID: 10913138
4. Determination of autoantibodies to annexin XI in systemic autoimmune diseases.  |  Jorgensen, CS., et al. 2000. Lupus. 9: 515-20. PMID: 11035417
5. S100A6, a calcium- and zinc-binding protein, is overexpressed in SOD1 mutant mice, a model for amyotrophic lateral sclerosis.  |  Hoyaux, D., et al. 2000. Biochim Biophys Acta. 1498: 264-72. PMID: 11108968
6. Regulation of cell specific expression of calcyclin (S100A6) in nerve cells and other tissues.  |  Leśniak, W., et al. 2000. Acta Neurobiol Exp (Wars). 60: 569-75. PMID: 11200185
7. Identification of a cell cycle-dependent gene product as a sialic acid-binding protein.  |  Gabius, HJ., et al. 1989. Biochem Biophys Res Commun. 163: 506-12. PMID: 2775283
8. Calcyclin-binding protein contributes to cholangiocarcinoma progression by inhibiting ubiquitination of MCM2.  |  Zhang, Y., et al. 2023. Oncol Res. 31: 317-331. PMID: 37305391
9. Molecular cloning of the cDNA for a growth factor-inducible gene with strong homology to S-100, a calcium-binding protein.  |  Calabretta, B., et al. 1986. J Biol Chem. 261: 12628-32. PMID: 3755724
10. S100A6: molecular function and biomarker role.  |  Wang, Y., et al. 2023. Biomark Res. 11: 78. PMID: 37670392
11. Calcyclin--from basic research to clinical implications.  |  Filipek, A. and Kuźnicki, J. 1993. Acta Biochim Pol. 40: 321-7. PMID: 8249488
12. Distribution of a specific calcium-binding protein of the S100 protein family, S100A6 (calcyclin), in subpopulations of neurons and glial cells of the adult rat nervous system.  |  Yamashita, N., et al. 1999. J Comp Neurol. 404: 235-57. PMID: 9934997

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Calcyclin Antibody (7D11A8) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Calcyclin Antibody (7D11A8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Calcyclin Antibody (7D11A8) Specifications

Alternate Product Name: Calcyclin

Product Clone Name: 7D11A8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Calcyclin

Epitope Species: human

Product Reactivity/Detection: Calcyclin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Calcyclin Antibody (7D11A8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Calcyclin antibody is Calcyclin Antibody (F-1): sc-271396.