CAF-1 p150 Antibody (p150-1): sc-56642

CAF-1 p150 Antibody (p150-1) is a mouse monoclonal IgG1 antibody that detects chromatin assembly factor-1 p150 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). CAF-1 p150 is a crucial component of the chromatin assembly factor-1 complex, which plays a vital role in the deposition of newly synthesized and acetylated histones H3 and H4 into nascent chromatin during DNA replication. This process is essential for maintaining chromatin structure and function, as chromatin assembly factor-1 p150 ensures that genetic material is properly packaged and accessible for transcription and replication. The p150 subunit not only facilitates the assembly of nucleosomes but also supports the maintenance of heterochromatin, which is characterized by its densely coiled structure and low gene density. Heterochromatin is critical for regulating gene expression and maintaining genomic stability, as heterochromatin contains large blocks of repetitive DNA that are less accessible to DNA-modifying enzymes. During late S phase, p150 interacts with heterochromatin-associated proteins, such as HP1α, HP1β, and HP1γ, which are integral to the structural integrity of heterochromatin. As cells transition into mitosis, the dynamic association and dissociation of p150 and HP1 proteins with chromatin are tightly regulated, coinciding with post-translational modifications of histones, such as the phosphorylation of histone H3. CAF-1 p150′s ability to interact with these proteins and participate in chromatin remodeling underscores CAF-1 p150′s importance in cellular processes, including gene regulation and DNA repair, making anti-CAF-1 p150 antibody (p150-1) an invaluable tool for researchers studying chromatin dynamics and epigenetic regulation.

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CAF-1 p150 Antibody (p150-1) References:

1. Molecular determinants for targeting heterochromatin protein 1-mediated gene silencing: direct chromoshadow domain-KAP-1 corepressor interaction is essential.  |  Lechner, MS., et al. 2000. Mol Cell Biol. 20: 6449-65. PMID: 10938122
2. A truncated form of the human CAF-1 p150 subunit impairs the maintenance of transcriptional gene silencing in mammalian cells.  |  Tchénio, T., et al. 2001. Mol Cell Biol. 21: 1953-61. PMID: 11238931
3. The replication kinase Cdc7-Dbf4 promotes the interaction of the p150 subunit of chromatin assembly factor 1 with proliferating cell nuclear antigen.  |  Gérard, A., et al. 2006. EMBO Rep. 7: 817-23. PMID: 16826239
4. The rice flattened shoot meristem, encoding CAF-1 p150 subunit, is required for meristem maintenance by regulating the cell-cycle period.  |  Abe, M., et al. 2008. Dev Biol. 319: 384-93. PMID: 18538315
5. Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites.  |  Zhu, Q., et al. 2009. DNA Repair (Amst). 8: 262-73. PMID: 19059499
6. Two fundamentally distinct PCNA interaction peptides contribute to chromatin assembly factor 1 function.  |  Rolef Ben-Shahar, T., et al. 2009. Mol Cell Biol. 29: 6353-65. PMID: 19822659
7. An analysis of CAF-1-interacting proteins reveals dynamic and direct interactions with the KU complex and 14-3-3 proteins.  |  Hoek, M., et al. 2011. J Biol Chem. 286: 10876-87. PMID: 21209461
8. CAF-1 Subunits Levels Suggest Combined Treatments with PARP-Inhibitors and Ionizing Radiation in Advanced HNSCC.  |  Morra, F., et al. 2019. Cancers (Basel). 11: PMID: 31627329
9. Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4.  |  Verreault, A., et al. 1996. Cell. 87: 95-104. PMID: 8858152
10. Nucleosome assembly activity and intracellular localization of human CAF-1 changes during the cell division cycle.  |  Marheineke, K. and Krude, T. 1998. J Biol Chem. 273: 15279-86. PMID: 9614144

The preferred CAF-1 p150 antibody is CAF-1 p150 Antibody (D-1): sc-133105.