C19orf46 Double Nickase Plasmid (h): sc-407273-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C19orf46 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C19orf46 Double Nickase Plasmid (h) and C19orf46 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SYNE4. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C19orf46 Double Nickase Plasmid (h) | sc-407273-NIC | 20 µg | $410.00 | |||
C19orf46 Double Nickase Plasmid (h2) | sc-407273-NIC-2 | 20 µg | $410.00 |
SYNE4 (also reported in some annotations as C19orf46) encodes Nesprin-4, an outer nuclear membrane KASH-domain protein that couples the nucleus to the cytoskeleton through LINC complexes with SUN proteins. This linkage helps coordinate nuclear positioning, mechanotransduction, and cytoskeletal organization, processes relevant to cell migration, polarity, and tissue architecture. SYNE4 is particularly studied in sensory epithelia where nucleus–cytoskeleton coupling supports cellular integrity, and disruption of LINC-mediated mechanics has been associated with neurosensory dysfunction. In cancer biology and genome stability research, altered nuclear mechanics and nuclear envelope protein networks are frequently examined as contributors to invasion phenotypes and stress responses.
C19orf46 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SYNE4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SYNE4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SYNE4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SYNE4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.