C14orf118 Double Nickase Plasmid (h): sc-412571-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C14orf118 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C14orf118 Double Nickase Plasmid (h) and C14orf118 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPATCH2L. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C14orf118 Double Nickase Plasmid (h) | sc-412571-NIC | 20 µg | $410.00 | |||
C14orf118 Double Nickase Plasmid (h2) | sc-412571-NIC-2 | 20 µg | $410.00 |
GPATCH2L (C14orf118) encodes a predicted G-patch domain–containing protein implicated in RNA biology, consistent with roles in RNA binding and modulation of RNA processing events. G-patch proteins frequently interface with RNA helicases and spliceosome-associated factors, linking them to pre-mRNA splicing, ribonucleoprotein complex dynamics, and broader control of gene expression. Although C14orf118 remains incompletely characterized, perturbation of RNA processing pathways can influence cell-cycle regulation, stress responses, and proteostasis through downstream transcriptome remodeling. As such, GPATCH2L is of interest for mechanistic studies connecting RNA metabolism to phenotypes relevant to cancer and neurodevelopmental or neurodegenerative disease models where splicing and RNA homeostasis are frequently altered.
C14orf118 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPATCH2L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPATCH2L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPATCH2L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPATCH2L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.