Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BMPR-IB Double Nickase Plasmid (m): sc-419351-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMPR-IB Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BMPR-IB Double Nickase Plasmid (m) and BMPR-IB Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Bmpr1b. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BMPR-IB Antibody (C-8): sc-515886
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMPR-IB Double Nickase Plasmid (m) | sc-419351-NIC | 20 µg | $410.00 |
Bmpr1b encodes the mouse bone morphogenetic protein receptor type IB (BMPR-IB), a serine/threonine kinase receptor that binds BMP ligands and initiates canonical SMAD1/5/8 signaling as well as non-canonical MAPK pathways. BMPR-IB regulates lineage specification, tissue morphogenesis, and homeostasis by integrating extracellular BMP cues into transcriptional programs controlling proliferation and differentiation. In developmental and reproductive biology, BMPR-IB is implicated in skeletal patterning and ovarian folliculogenesis, and altered BMP/BMPR-IB signaling has been linked to dysregulated differentiation and remodeling in multiple organ systems. As a pathway node, BMPR-IB is frequently studied for its role in crosstalk with TGF-β family signaling and context-dependent control of cell fate decisions.
BMPR-IB Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Bmpr1b locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Bmpr1b. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Bmpr1b function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Bmpr1b-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.