BDNF Double Nickase Plasmid (h): sc-400029-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BDNF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BDNF Double Nickase Plasmid (h) and BDNF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BDNF. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: pro BDNF Antibody (5H8): sc-65514
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BDNF Double Nickase Plasmid (h) | sc-400029-NIC | 20 µg | $410.00 | |||
BDNF Double Nickase Plasmid (h2) | sc-400029-NIC-2 | 20 µg | $410.00 |
Brain-derived neurotrophic factor (BDNF) is a secreted neurotrophin that signals primarily through NTRK2/TrkB and p75NTR to regulate neuronal survival, differentiation, synaptic plasticity, and activity-dependent circuit remodeling. BDNF-driven signaling engages MAPK/ERK, PI3K–AKT, and PLCγ pathways, influencing neurite outgrowth, long-term potentiation, and transcriptional programs linked to learning and memory. Altered BDNF expression or signaling has been associated with neuropsychiatric and neurodegenerative disease mechanisms, including stress responsivity, mood regulation, and impaired synaptic function. In addition to the nervous system, BDNF is studied in neuroimmune crosstalk and metabolic regulation, supporting broad utility in mechanistic research models.
BDNF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BDNF locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BDNF. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BDNF function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BDNF-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.