apoC-III Double Nickase Plasmid (m): sc-419165-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- apoC-III Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- apoC-III Double Nickase Plasmid (m) and apoC-III Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Apoc3. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
apoC-III Double Nickase Plasmid (m) | sc-419165-NIC | 20 µg | $410.00 |
Mouse Apoc3 encodes apolipoprotein C-III (apoC-III), a secreted apolipoprotein that associates with triglyceride-rich lipoproteins and modulates plasma lipid handling. apoC-III influences lipoprotein lipase–dependent triglyceride hydrolysis and hepatic clearance of remnant particles, linking it to lipid transport, energy metabolism, and endocrine regulation of fasting–feeding responses. Altered Apoc3 expression impacts systemic triglyceride homeostasis and is frequently studied in the context of dyslipidemia-associated metabolic phenotypes, including insulin resistance and fatty liver–related processes. In mice, Apoc3 is also used as a model locus to interrogate hepatocyte secretory pathways, lipoprotein remodeling, and gene–diet interactions.
apoC-III Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Apoc3 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Apoc3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Apoc3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Apoc3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.