AP4A Hydrolase Double Nickase Plasmid (h): sc-407028-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AP4A Hydrolase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AP4A Hydrolase Double Nickase Plasmid (h) and AP4A Hydrolase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NUDT2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AP4A Hydrolase Antibody (F-5): sc-271410
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AP4A Hydrolase Double Nickase Plasmid (h) | sc-407028-NIC | 20 µg | $410.00 | |||
AP4A Hydrolase Double Nickase Plasmid (h2) | sc-407028-NIC-2 | 20 µg | $410.00 |
NUDT2 encodes human Ap4A hydrolase, a Nudix family enzyme that hydrolyzes diadenosine tetraphosphate (Ap4A) and related dinucleoside polyphosphates to regulate intracellular “alarmone” signaling. By controlling Ap4A turnover, NUDT2 influences nucleotide homeostasis and stress-responsive pathways that affect transcriptional programs, RNA metabolism, and cell-cycle progression. Altered NUDT2 activity has been linked in the literature to changes in proliferative capacity, DNA damage responses, and inflammatory signaling networks, making it relevant for studying context-dependent remodeling of signaling cascades in human cells. As Ap4A levels can modulate protein–nucleotide interactions and downstream kinase/adapter signaling, NUDT2 perturbation is useful for dissecting nucleotide-mediated control of cellular state transitions.
AP4A Hydrolase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NUDT2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NUDT2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NUDT2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NUDT2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.