Ankyrin G Antibody (3G8): sc-70359

Ankyrin G Antibody (3G8) is a mouse monoclonal IgG1 antibody that detects Ankyrin G in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Ankyrin G is a crucial member of the Ankyrin family of proteins, which play a vital role in linking integral membrane proteins to the cytoskeleton, thereby maintaining cellular structure and function. Anti-Ankyrin G antibody (3G8) recognizes a protein predominantly localized to the nodes of Ranvier and axon initial segments in neurons, where Ankyrin G is essential for the proper functioning of sodium channels, facilitating the rapid propagation of action potentials. Ankyrin G′s location in these critical sites ensures efficient neuronal signaling and communication. Additionally, Ankyrin G exhibits tissue-specific alternative mRNA processing, resulting in various isoforms that are predominantly expressed in nervous tissue, while smaller isoforms are found in other organs such as the lung, kidney, spleen, liver, and heart. The diverse expression patterns and functional roles of Ankyrin G underscore its importance in both neuronal health and overall cellular integrity, making Ankyrin G monoclonal antibody (3G8) an invaluable tool for researchers studying these processes.

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Ankyrin G Antibody (3G8) References:

1. Ankyrin-G palmitoylation and βII-spectrin binding to phosphoinositide lipids drive lateral membrane assembly.  |  He, M., et al. 2014. J Cell Biol. 206: 273-88. PMID: 25049274
2. Ankyrin-G Inhibits Endocytosis of Cadherin Dimers.  |  Cadwell, CM., et al. 2016. J Biol Chem. 291: 691-704. PMID: 26574545
3. NF-κB regulates neuronal ankyrin-G via a negative feedback loop.  |  König, HG., et al. 2017. Sci Rep. 7: 42006. PMID: 28181483
4. Homer1 promotes dendritic spine growth through ankyrin-G and its loss reshapes the synaptic proteome.  |  Yoon, S., et al. 2021. Mol Psychiatry. 26: 1775-1789. PMID: 33398084
5. Giant ankyrin-G regulates cardiac function.  |  Cavus, O., et al. 2021. J Biol Chem. 296: 100507. PMID: 33675749
6. Ankyrin-G Heterozygous Knockout Mice Display Increased Sensitivity to Social Defeat Stress.  |  Cordner, ZA., et al. 2021. Complex Psychiatry. 7: 71-79. PMID: 35928299
7. Reduced expression of Ankyrin-G and E-cadherin in duodenal mucosal biopsy of subjects with celiac disease.  |  Sharma, N., et al. 2024. Pathol Res Pract. 255: 155164. PMID: 38324966
8. Live imaging of excitable axonal microdomains in ankyrin-G-GFP mice.  |  Thome, C., et al. 2024. bioRxiv.. PMID: 38948770
9. An ankyrin G-binding motif mediates TRAAK periodic localization at axon initial segments of hippocampal pyramidal neurons.  |  Luque-Fernández, V., et al. 2024. Proc Natl Acad Sci U S A. 121: e2310120121. PMID: 39058579
10. Regulation of Ankyrin-G on Nav1.5 Channel in Hypoxic HL-1 Cardiac Muscle Cells.  |  Ma, S., et al. 2024. Discov Med. 36: 2191-2201. PMID: 39600274

The preferred Ankyrin G antibody is Ankyrin G Antibody (463): sc-12719.