Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Angiopoietin 2/Ang-2/ANGPT2 Double Nickase Plasmid (h): sc-400477-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Angiopoietin 2/Ang-2/ANGPT2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Angiopoietin 2/Ang-2/ANGPT2 Double Nickase Plasmid (h) and Angiopoietin 2/Ang-2/ANGPT2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ANGPT2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Angiopoietin 2/Ang-2/ANGPT2 Antibody (F-1): sc-74403
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Angiopoietin 2/Ang-2/ANGPT2 Double Nickase Plasmid (h) | sc-400477-NIC | 20 µg | $410.00 | |||
Angiopoietin 2/Ang-2/ANGPT2 Double Nickase Plasmid (h2) | sc-400477-NIC-2 | 20 µg | $410.00 |
ANGPT2 encodes angiopoietin-2 (Ang-2), a secreted ligand for the endothelial receptor tyrosine kinase TEK/TIE2 that modulates vascular remodeling, endothelial activation, and permeability. In contrast to ANGPT1-mediated stabilization, Ang-2 often functions as a context-dependent antagonist of TIE2 signaling, sensitizing endothelium to inflammatory cytokines and VEGF-driven angiogenic cues. ANGPT2 integrates with PI3K–AKT, MAPK, and NF-κB-linked programs to influence sprouting, junction dynamics, and leukocyte trafficking. Dysregulated ANGPT2 expression is frequently associated with pathological angiogenesis and vascular inflammation observed in tumors, retinopathies, and cardiometabolic and inflammatory disorders, supporting its use as a mechanistic node in vascular biology research.
Angiopoietin 2/Ang-2/ANGPT2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ANGPT2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ANGPT2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ANGPT2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ANGPT2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.