Amelogenin Double Nickase Plasmid (h): sc-403337-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Amelogenin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Amelogenin Double Nickase Plasmid (h) and Amelogenin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AMELY. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Amelogenin Antibody (F-11): sc-365284
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Amelogenin Double Nickase Plasmid (h) | sc-403337-NIC | 20 µg | $410.00 | |||
Amelogenin Double Nickase Plasmid (h2) | sc-403337-NIC-2 | 20 µg | $410.00 |
AMELY encodes the Y-chromosome amelogenin isoform, a secreted extracellular matrix protein that contributes to enamel biomineralization and tooth development through regulation of hydroxyapatite crystal nucleation, growth, and matrix organization. Amelogenin participates in enamel matrix assembly and proteolytic processing during amelogenesis, influencing epithelial–mesenchymal interactions and mineral deposition programs. Genetic variation affecting amelogenin expression or structure has been linked to enamel formation defects and phenotypes within amelogenesis imperfecta–spectrum disorders, supporting its relevance to craniofacial and dental developmental biology. Because AMELY is Y-linked and closely related to AMELX, it is also useful for studying sex chromosome biology and paralog-specific regulation in odontogenic models.
Amelogenin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AMELY locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AMELY. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AMELY function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AMELY-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.