ALB/Albumin Double Nickase Plasmid (h): sc-400269-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ALB/Albumin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ALB/Albumin Double Nickase Plasmid (h) and ALB/Albumin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ALB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ALB/Albumin Antibody (F-10): sc-271605
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ALB/Albumin Double Nickase Plasmid (h) | sc-400269-NIC | 20 µg | $410.00 | |||
ALB/Albumin Double Nickase Plasmid (h2) | sc-400269-NIC-2 | 20 µg | $410.00 |
Human ALB encodes albumin, the most abundant plasma protein and a principal determinant of oncotic pressure, acting as a major carrier for fatty acids, steroid hormones, bilirubin, and diverse xenobiotics. Predominantly synthesized and secreted by hepatocytes, albumin contributes to systemic nutrient distribution, redox buffering through its free thiol, and modulation of ligand bioavailability in circulation. ALB expression is tightly linked to hepatocyte differentiation, liver metabolic programs, and secretory pathway function, making it a widely used marker in studies of hepatic identity and maturation. Altered albumin production or distribution is associated with liver dysfunction and systemic inflammatory states, supporting its relevance in modeling hepatic stress responses and protein homeostasis.
ALB/Albumin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ALB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ALB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ALB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ALB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.