Aiolos Double Nickase Plasmid (h): sc-400764-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aiolos Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Aiolos Double Nickase Plasmid (h) and Aiolos Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IKZF3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aiolos Antibody (3H5-G7): sc-293421
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aiolos Double Nickase Plasmid (h) | sc-400764-NIC | 20 µg | $410.00 | |||
Aiolos Double Nickase Plasmid (h2) | sc-400764-NIC-2 | 20 µg | $410.00 |
IKZF3 encodes Aiolos, a zinc finger transcription factor of the Ikaros family that regulates gene expression programs required for lymphocyte maturation, immune tolerance, and B cell differentiation. Aiolos integrates with chromatin remodeling and transcriptional repression/activation complexes to modulate signaling outputs downstream of antigen receptor stimulation and cytokine-driven pathways. Dysregulated IKZF3 activity has been linked to altered lymphocyte homeostasis, aberrant antibody responses, and immune-mediated pathophysiology, making it a useful node for studying transcriptional control in hematopoietic lineages. As a nuclear regulator, Aiolos is frequently examined in the context of epigenetic regulation, lineage commitment, and immune cell signaling networks.
Aiolos Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IKZF3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IKZF3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IKZF3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IKZF3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.