AICAR Double Nickase Plasmid (h): sc-404771-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AICAR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AICAR Double Nickase Plasmid (h) and AICAR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATIC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AICAR transformylase Antibody (F38 P7 H9): sc-53612
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AICAR Double Nickase Plasmid (h) | sc-404771-NIC | 20 µg | $410.00 | |||
AICAR Double Nickase Plasmid (h2) | sc-404771-NIC-2 | 20 µg | $410.00 |
Adenosine monophosphate deaminase–interacting protein encoded by human ATIC (AICAR transformylase/IMP cyclohydrolase) is a bifunctional enzyme catalyzing the final two steps of de novo purine biosynthesis, converting AICAR to IMP via 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and IMP cyclohydrolase activities. By controlling IMP production, ATIC helps maintain nucleotide pools required for DNA/RNA synthesis and supports energy homeostasis in proliferating cells. ATIC activity intersects with one-carbon/folate-dependent metabolism through its formyltransferase reaction, linking purine synthesis to methyl-group supply and redox balance. Dysregulation of purine and folate pathways is frequently studied in the context of metabolic stress, genome stability, and altered proliferation programs relevant to cancer biology and inborn errors of purine metabolism research.
AICAR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATIC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATIC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATIC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATIC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.