Adenosine A2A-R Double Nickase Plasmid (h): sc-400807-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Adenosine A2A-R Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Adenosine A2A-R Double Nickase Plasmid (h) and Adenosine A2A-R Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADORA2A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Adenosine A2A-R Antibody (7F6-G5-A2): sc-32261
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Adenosine A2A-R Double Nickase Plasmid (h) | sc-400807-NIC | 20 µg | $410.00 | |||
Adenosine A2A-R Double Nickase Plasmid (h2) | sc-400807-NIC-2 | 20 µg | $410.00 |
ADORA2A encodes the human adenosine A2A receptor (Adenosine A2A-R), a Gs-coupled GPCR that elevates intracellular cAMP and activates PKA/CREB-dependent transcription in response to extracellular adenosine. This signaling axis modulates neurotransmission, vasodilation, and immune cell function, integrating metabolic stress cues into cellular responses. A2A-R cross-talks with dopaminergic and glutamatergic pathways and shapes inflammatory signaling through cAMP-mediated regulation of cytokine production and T cell activity. Dysregulated ADORA2A signaling has been implicated in neurodegenerative and neuropsychiatric disorders, tumor microenvironment–associated immune suppression, and cardiopulmonary inflammatory processes, supporting its use as a mechanistic target in disease-relevant models.
Adenosine A2A-R Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADORA2A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADORA2A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADORA2A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADORA2A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.