ACP1 Double Nickase Plasmid (h): sc-404488-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ACP1 Double Nickase Plasmid (h) and ACP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ACP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACP1 Antibody (D-3): sc-390190
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACP1 Double Nickase Plasmid (h) | sc-404488-NIC | 20 µg | $410.00 | |||
ACP1 Double Nickase Plasmid (h2) | sc-404488-NIC-2 | 20 µg | $410.00 |
Human ACP1 encodes a low-molecular-weight protein tyrosine phosphatase (LMW-PTP) that dephosphorylates phosphotyrosine residues on key signaling proteins, shaping the amplitude and duration of receptor-initiated pathways. By modulating phosphorylation-dependent events, ACP1 influences growth factor receptor signaling, cytoskeletal dynamics, and downstream MAPK and PI3K/AKT network behavior that govern proliferation, adhesion, and metabolic responses. Genetic and functional studies have linked ACP1 activity and variation to altered immune-cell signaling and metabolic phenotypes, supporting its relevance in research on inflammation, insulin signaling, and oncogenic pathway rewiring. As a phosphatase node that counterbalances kinase activity, ACP1 is frequently interrogated in studies of signal transduction fidelity and phosphorylation homeostasis.
ACP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ACP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ACP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ACP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ACP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.