Ac-Histone H2B Antibody (E-6): sc-515937

Ac-Histone H2B Antibody (E-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects Ac-Histone H2B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Ac-Histone H2B antibody (E-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ac-Histone H2B protein plays a crucial role in regulating gene expression through involvement in chromatin structure and dynamics. Specifically, acetylation of histones, including H2B, is a key post-translational modification that alters chromatin accessibility, thereby influencing binding of transcription factors and transcriptional machinery to DNA. This modification is essential for activating gene transcription, as acetylation reduces positive charge on histones, leading to a more relaxed chromatin structure that allows greater accessibility to DNA. Dynamic regulation of histone acetylation is mediated by various enzymes, including histone acetyltransferases and deacetylases, which play significant roles in cellular processes such as differentiation, proliferation, and response to environmental signals. Understanding Ac-Histone H2B function and acetylation status is vital for elucidating mechanisms of gene regulation and potential implications in diseases such as cancer, where dysregulation of histone modifications can lead to aberrant gene expression.

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Ac-Histone H2B Antibody (E-6) References:

1. Acetylation of histone H2B mirrors that of H4 and H3 at the chicken beta-globin locus but not at housekeeping genes.  |  Myers, FA., et al. 2003. J Biol Chem. 278: 36315-22. PMID: 12865423
2. Apoptosis-associated acetylation on histone H2B is an epitope for lupus autoantibodies.  |  van Bavel, CC., et al. 2009. Mol Immunol. 47: 511-6. PMID: 19747733
3. Extensive and varied modifications in histone H2B of wild-type and histone deacetylase 1 mutant Neurospora crassa.  |  Anderson, DC., et al. 2010. Biochemistry. 49: 5244-57. PMID: 20462202
4. Multi-faceted quantitative proteomics analysis of histone H2B isoforms and their modifications.  |  Molden, RC., et al. 2015. Epigenetics Chromatin. 8: 15. PMID: 25922622
5. Histone H2B-IFI16 Recognition of Nuclear Herpesviral Genome Induces Cytoplasmic Interferon-β Responses.  |  Iqbal, J., et al. 2016. PLoS Pathog. 12: e1005967. PMID: 27764250
6. Repression of the PRELP gene is relieved by histone deacetylase inhibitors through acetylation of histone H2B lysine 5 in bladder cancer.  |  Shozu, K., et al. 2022. Clin Epigenetics. 14: 147. PMID: 36371227
7. The anaphase promoting complex/cyclosome ubiquitylates histone H2B on the promoter during UbcH10 transactivation.  |  Chakraborty, P., et al. 2023. FEBS Lett. 597: 437-447. PMID: 36520528
8. Acetylation of histone H2B marks active enhancers and predicts CBP/p300 target genes.  |  Narita, T., et al. 2023. Nat Genet. 55: 679-692. PMID: 37024579
9. The Role of Histone H2B Acetylation Modification in Aluminum-Induced Cognitive Dysfunction.  |  Gao, J., et al. 2024. Biol Trace Elem Res. 202: 3731-3739. PMID: 37979070
10. Histone H2B lysine 122 and lysine 130, as the putative targets of Penicillium oxalicum LaeA, play important roles in asexual development, expression of secondary metabolite gene clusters, and extracellular glycoside hydrolase synthesis.  |  Zhang, X., et al. 2024. World J Microbiol Biotechnol. 40: 179. PMID: 38668807

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ac-Histone H2B Antibody (E-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Ac-Histone H2B Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ac-Histone H2B Antibody (E-6) Specifications

Alternate Product Name: Histone acetylation H2B

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-28

Epitope Target: Histone H2B

Epitope Species: human

Product Reactivity/Detection: Histone H2B acetylated at Lys 5, Lys 12, Lys 15 and Lys 20

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Ac-Histone H2B Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Histone H2B antibody is Histone H2B Antibody (A-6): sc-515808.