AANAT CRISPR/Cas9 KO Plasmid (m): sc-418927
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AANAT CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AANAT genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AANAT CRISPR/Cas9 KO Plasmid (m) | sc-418927 | 20 µg | $397.00 |
Overview
Mouse Aanat encodes arylalkylamine N-acetyltransferase (AANAT), a rate-limiting enzyme in melatonin biosynthesis that catalyzes N-acetylation of serotonin to N-acetylserotonin in the pineal gland and other tissues. AANAT activity is tightly regulated by circadian cues through cAMP/PKA signaling, phosphorylation-dependent stabilization, and coordinated transcriptional control, linking cellular metabolism to light–dark cycle entrainment. By modulating melatonin and related indoleamine intermediates, Aanat influences sleep–wake regulation, seasonal physiology, and neuroendocrine signaling. Dysregulated AANAT expression or activity has been associated in experimental systems with circadian rhythm disruption and altered stress, mood, and metabolic phenotypes, making it a useful node for pathway-focused studies.
AANAT CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Aanat gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Aanat together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Aanat open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AANAT protein expression.
This CRISPR knockout system enables efficient generation of Aanat-deficient cell models for investigation of AANAT signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Aanat exon(s) critical for AANAT function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Aanat genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AANAT CRISPR/Cas9 KO Plasmid (m) and AANAT CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Aanat locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AANAT HDR Plasmid (m) and AANAT HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Aanat homology arms to support homology-directed repair at defined Aanat target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.