Molecular structure of 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, CAS Number: 7240-90-6
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside (CAS 7240-90-6)
See product citations (4)
Alternate Names:
X-Gal; BCIG; 5-Bromo-4-chloro-3-indolyl-β-D-galactoside; X Gal
Application:
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is a chromogenic histochemical substrate for β-galactosidase
CAS Number:
7240-90-6
Purity:
≥99%
Molecular Weight:
408.63
Molecular Formula:
C14H15BrClNO6
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal) is a histochemical substrate for beta-galactosidase. 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is cleaved by beta-galactosidase to yield an insoluble blue preceipitate. It is used to detect DNA insertions into the lacZ region of plasmid DNA, which causes the loss of beta-galactosidase activity. It is also used in conjunction with IPTG for detection of b-Galactosidase activity in bacterial colonies in a colorimetric assay.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside (CAS 7240-90-6) References
- Use of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside for the isolation of beta-galactosidase-positive bacteria from municipal water supplies. | Ley, A., et al. 1993. Can J Microbiol. 39: 821-5. PMID: 10755843
- Evaluation of p-naphtholbenzein-beta-D-galactoside as a substrate for bacterial beta-galactosidase. | James, AL., et al. 2000. Appl Environ Microbiol. 66: 5521-3. PMID: 11097942
- Purification and some characteristics of a recombinant dimeric rhizobium meliloti beta-galactosidase expressed in escherichia coli. | Leahy, M., et al. 2001. Enzyme Microb Technol. 28: 682-688. PMID: 11339953
- A fluorimetric method using fluorescein di-beta-D-galactopyranoside for quantifying the senescence-associated beta-galactosidase activity in human foreskin fibroblast Hs68 cells. | Yang, NC. and Hu, ML. 2004. Anal Biochem. 325: 337-43. PMID: 14751269
- New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria. | Arnaud, M., et al. 2004. Appl Environ Microbiol. 70: 6887-91. PMID: 15528558
- Quantitative assay of senescence-associated beta-galactosidase activity in mammalian cell extracts. | Gary, RK. and Kindell, SM. 2005. Anal Biochem. 343: 329-34. PMID: 16004951
- Chemiluminescent assay of various enzymes using indoxyl derivatives as substrate and its applications to enzyme immunoassay and DNA probe assay. | Arakawa, H., et al. 1991. Anal Biochem. 199: 238-42. PMID: 1725950
- Improved methods for the detection of beta-galactosidase activity in colonies of Escherichia coli using a new chromogenic substrate: VBzTM-gal (2-(2-(4-(beta-D-galactopyranosyloxy)-3-methoxyphenyl)-vinyl)-3- methylbenzothiazolium toluene-4-sulphonate). | Bainbridge, BW., et al. 1991. FEMS Microbiol Lett. 64: 319-23. PMID: 1909279
- X-gal staining of the central nervous system in adult Drosophila. | Sweeney, ST., et al. 2012. Cold Spring Harb Protoc. 2012: 239-41. PMID: 22301646
- Intermittent parathyroid hormone administration converts quiescent lining cells to active osteoblasts. | Kim, SW., et al. 2012. J Bone Miner Res. 27: 2075-84. PMID: 22623172
- Production of 2-aminophenoxazin-3-one by Staphylococcus aureus causes false-positive results in β-galactosidase assays. | Tse, H., et al. 2012. J Clin Microbiol. 50: 3780-2. PMID: 22972831
- Analysis of intracellular enzyme activity by surface enhanced Raman scattering. | Stevenson, R., et al. 2013. Analyst. 138: 6331-6. PMID: 24003438
- Comments on Methods to Suppress Endogenous β-Galactosidase Activity in Mouse Tissues Expressing the LacZ Reporter Gene. | Merkwitz, C., et al. 2016. J Histochem Cytochem. 64: 579-86. PMID: 27555495
- A double staining technique using 5-bromo,4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) and immunoperoxidase in whole Drosophila embryos. | Kobayashi, S. and Okada, M. 1993. Biotech Histochem. 68: 237-9. PMID: 8218577
- Chemiluminescent assay of beta-D-galactosidase based on indole luminescence. | Arakawa, H., et al. 1998. J Biolumin Chemilumin. 13: 349-54. PMID: 9926362
Substrate of:
beta-Galactosidase, and Chromogenic.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, 1 g | sc-280488A | 1 g | $90.00 | |||
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, 5 g | sc-280488 | 5 g | $210.00 | |||
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, 25 g | sc-280488B | 25 g | $815.00 | |||
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, 100 g | sc-280488C | 100 g | $2920.00 |