14-3-3 θ Antibody (5J20): sc-69720

14-3-3 θ Antibody (5J20) is a mouse monoclonal IgG1 kappa light chain antibody that detects 14-3-3 theta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-14-3-3 theta antibody (5J20) is available as non-conjugated format. 14-3-3 theta protein plays a crucial role in regulating various cellular processes, particularly in cancer biology, where 14-3-3 theta protein is involved in apoptosis, mitogenic signaling, and cell-cycle checkpoints. 14-3-3 theta protein is primarily located in the cytoplasm, where 14-3-3 theta protein interacts with a wide range of partner proteins, facilitating proper function and localization. Cytoplasmic presence of 14-3-3 theta protein allows regulation of signaling pathways essential for cell survival and proliferation. Through sequestering target proteins in the cytoplasm, 14-3-3 theta protein prevents degradation and modulates activity, influencing critical cellular responses to stress and growth signals. Formation of dimers by 14-3-3 proteins creates binding sites for ligands sharing consensus binding motifs, underscoring importance in maintaining cellular homeostasis. Loss of expression or dysfunction of 14-3-3 theta protein can lead to dysregulation of these pathways, contributing to various pathological conditions, including cancer.

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14-3-3 θ Antibody (5J20) References:

1. The 4.1/ezrin/radixin/moesin domain of the DAL-1/Protein 4.1B tumour suppressor interacts with 14-3-3 proteins.  |  Yu, T., et al. 2002. Biochem J. 365: 783-9. PMID: 11996670
2. MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1.  |  Lim, R., et al. 2002. J Biol Chem. 277: 40997-1008. PMID: 12176995
3. The 14-3-3 cancer connection.  |  Hermeking, H. 2003. Nat Rev Cancer. 3: 931-43. PMID: 14737123
4. Isoform-specific subcellular localization among 14-3-3 proteins in Arabidopsis seems to be driven by client interactions.  |  Paul, AL., et al. 2005. Mol Biol Cell. 16: 1735-43. PMID: 15659648
5. Overexpression of 14-3-3θ promotes tumor metastasis and indicates poor prognosis in breast carcinoma.  |  Li, N., et al. 2014. Oncotarget. 5: 249-57. PMID: 24371149
6. MiR-27a downregulates 14-3-3θ, RUNX1, AF4, and MLL-AF4, crucial drivers of blast transformation in t(4;11) leukemia cells.  |  Fioretti, T., et al. 2022. Cell Biochem Funct. 40: 706-717. PMID: 35981137
7. Sinomenine attenuated dextran sulfate sodium-induced inflammatory responses by promoting 14-3-3θ protein and inhibiting NF-κB signaling.  |  Zhou, Y., et al. 2023. J Ethnopharmacol. 303: 116037. PMID: 36526094
8. 14-3-3θ phosphorylation exacerbates alpha-synuclein aggregation and toxicity.  |  Wang, B., et al. 2025. Neurobiol Dis. 206: 106801. PMID: 39805369
9. 14-3-3: modulators of signaling proteins?  |  Morrison, D. 1994. Science. 266: 56-7. PMID: 7939645
10. Structural organization and chromosomal assignment of the human 14-3-3 eta chain gene (YWHAH).  |  Muratake, T., et al. 1996. Genomics. 36: 63-9. PMID: 8812417
11. The structural basis for 14-3-3:phosphopeptide binding specificity.  |  Yaffe, MB., et al. 1997. Cell. 91: 961-71. PMID: 9428519
12. A novel sphingosine-dependent protein kinase (SDK1) specifically phosphorylates certain isoforms of 14-3-3 protein.  |  Megidish, T., et al. 1998. J Biol Chem. 273: 21834-45. PMID: 9705322

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-14-3-3 θ Antibody (5J20) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-14-3-3 θ Antibody (5J20) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

14-3-3 θ Antibody (5J20) Specifications

Alternate Product Name: 14-3-3 theta

Product Clone Name: 5J20

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: 14-3-3 θ

Epitope Species: human

Product Reactivity/Detection: 14-3-3 θ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for 14-3-3 θ Antibody (5J20): N/A