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ZKSCAN3 抗体 (A-10): sc-515285

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说明书
  • ZKSCAN3 抗体 A-10 是小鼠单克隆 IgG1 κ,ZKSCAN3抗体, 在5篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列442-491位于human物种的ZKSCAN3的C-terminus附近
  • ZKSCAN3 抗体 (A-10) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 ZKSCAN3
  • 抗ZKSCAN3抗体(A-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是ZKSCAN3 Antibody (A-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ZKSCAN3 Antibody (A-10) 打包提供(请参阅下面的订购信息)。
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関連項目

ZKSCAN3抗体(A-10)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测人类来源的ZKSCAN3。ZKSCAN3 (A-10) 抗体有非结合型和多种结合型可供选择,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。ZKSCAN3,又称KRAB和SCAN域锌指蛋白3,是一种由583个氨基酸组成的蛋白质,在转录调控中发挥着关键作用,特别是在癌症生物学领域。作为克鲁佩尔C2H2型锌指蛋白家族的一员,ZKSCAN3具有七个C2H2型锌指、一个KRAB结构域和一个SCAN结构域,这些结构域对于DNA结合能力和转录调控功能至关重要。ZKSCAN3与结直肠癌有关,研究表明,其过度表达与侵袭性肿瘤类型有关,并且编码ZKSCAN3的染色体区域在结直肠癌病例中经常被放大。ZKSCAN3 mRNA的敲除与结肠癌细胞系中无锚定依赖性生长受损和肿瘤生长减少有关,这凸显了其作为治疗靶标的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ZKSCAN3 抗体 (A-10) 参考文献:

  1. 人类高度保守锌指模块的计算机序列分析。  |  Petroni, D., et al. 1998. DNA Seq. 9: 163-9. PMID: 10520746
  2. SCAN 结构域定义了一个庞大的锌指转录因子家族。  |  Sander, TL., et al. 2003. Gene. 310: 29-38. PMID: 12801630
  3. 含 KRAB 域锌指蛋白的鉴定和特征描述。  |  Constantinou-Deltas, CD., et al. 1992. Genomics. 12: 581-9. PMID: 1559709
  4. 锌指转录因子的 SCAN 结构域家族。  |  Edelstein, LC. and Collins, T. 2005. Gene. 359: 1-17. PMID: 16139965
  5. 通过基于细胞的筛选,从表达的 cDNA ORF 文库中鉴定出五个与细胞增殖相关的人类新基因。  |  Ma, X., et al. 2007. Life Sci. 81: 1141-51. PMID: 17868742
  6. 以前未曾描述过的 ZKSCAN3 (ZNF306) 是结直肠癌进展的新型 '驱动因子'。  |  Yang, L., et al. 2008. Cancer Res. 68: 4321-30. PMID: 18519692
  7. Krüppel相关盒是有效的转录抑制结构域。  |  Margolin, JF., et al. 1994. Proc Natl Acad Sci U S A. 91: 4509-13. PMID: 8183939

订购信息

产品名称产品编号规格价格数量收藏夹

ZKSCAN3 抗体 (A-10)

sc-515285
200 µg/ml
$322.00

ZKSCAN3 (A-10): m-IgG Fc BP-HRP 套装

sc-531329
200 µg Ab; 10 µg BP
$361.00

ZKSCAN3 (A-10): m-IgGκ BP-HRP 套装

sc-524993
200 µg Ab, 40 µg BP
$361.00

ZKSCAN3 (A-10): m-IgG1 BP-HRP 套装

sc-544487
200 µg Ab; 20 µg BP
$361.00

ZKSCAN3 抗体 (A-10) AC

sc-515285 AC
500 µg/ml, 25% agarose
$424.00

ZKSCAN3 抗体 (A-10) HRP

sc-515285 HRP
200 µg/ml
$322.00

ZKSCAN3 抗体 (A-10) FITC

sc-515285 FITC
200 µg/ml
$336.00

ZKSCAN3 抗体 (A-10) PE

sc-515285 PE
200 µg/ml
$349.00

ZKSCAN3 抗体 (A-10) Alexa Fluor® 488

sc-515285 AF488
200 µg/ml
$364.00

ZKSCAN3 抗体 (A-10) Alexa Fluor® 546

sc-515285 AF546
200 µg/ml
$364.00

ZKSCAN3 抗体 (A-10) Alexa Fluor® 594

sc-515285 AF594
200 µg/ml
$364.00

ZKSCAN3 抗体 (A-10) Alexa Fluor® 647

sc-515285 AF647
200 µg/ml
$364.00

ZKSCAN3 抗体 (A-10) Alexa Fluor® 680

sc-515285 AF680
200 µg/ml
$364.00

ZKSCAN3 抗体 (A-10) Alexa Fluor® 790

sc-515285 AF790
200 µg/ml
$364.00

Does your ZKSCAN3 (A10) SC-515285 Ab cross react with mouse samples?

Asked by: Gerardo
Thank you for your question. This antibody has not yet been tested in mouse samples, and so it is not currently recommended for detection in mouse.
Answered by: Technical Support
Date published: 2019-05-11

What is the recommended starting dilution for Western blot for sc-515285: ZKSCAN3 (A-10) monoclonal antibody?

Asked by: Trav11
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
  • y_2026, m_5, d_24, h_14CST
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Rated 5 out of 5 by from Good for Western Blot[Employee of scbt] Antibody works for Western blot with mouse lung homogenates mice subjected to intratracheal (i.t.) instillation of LPS for 24 hours, or after exposure to P. aeruginosa strain K (PAK) for 4 hours in published research. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from Not that clear but good1:500 dilution, and use Oddyssey to scan it, you have to modify the contrast to see the band clearly.
Date published: 2018-05-02
Rated 5 out of 5 by from Nice features.The background is relatively clean and the sensitivity is OK. ( 1:1000 dilution was used )
Date published: 2017-10-01
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ZKSCAN3 抗体 (A-10) is rated 4.7 out of 5 by 3.
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