Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ZHX2 CRISPR/Cas9 KO Plasmid (m): sc-436490
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZHX2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZHX2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZHX2 Antibody (D-2): sc-393399
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZHX2 CRISPR/Cas9 KO Plasmid (m) | sc-436490 | 20 µg | $397.00 |
Overview
Zhx2 encodes the zinc fingers and homeoboxes protein 2 (ZHX2), a nuclear transcription factor that can act as a context-dependent regulator of gene expression through DNA binding and interactions with other transcriptional repressors. In mouse tissues, ZHX2 has been implicated in hepatocyte differentiation and maturation programs and in the control of liver-enriched metabolic gene networks, including pathways linked to lipid handling and xenobiotic processing. Altered ZHX2 activity has been associated with dysregulated transcriptional programs relevant to inflammation and tumor biology, making it a useful node for studying transcriptional control of tissue homeostasis. These properties support investigation of ZHX2-dependent chromatin and transcriptional mechanisms in development and disease-relevant cellular states.
ZHX2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Zhx2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Zhx2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Zhx2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZHX2 protein expression.
This CRISPR knockout system enables efficient generation of Zhx2-deficient cell models for investigation of ZHX2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Zhx2 exon(s) critical for ZHX2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Zhx2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZHX2 CRISPR/Cas9 KO Plasmid (m) and ZHX2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Zhx2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZHX2 HDR Plasmid (m) and ZHX2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Zhx2 homology arms to support homology-directed repair at defined Zhx2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.