ZCCHC6 CRISPR/Cas9 KO Plasmid (h): sc-418151
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZCCHC6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZCCHC6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZCCHC6 CRISPR/Cas9 KO Plasmid (h) | sc-418151 | 20 µg | $397.00 |
Overview
ZCCHC6 (also known as TUT7) encodes a zinc finger CCHC-type–containing terminal uridylyltransferase that catalyzes 3′ uridylation of diverse RNA substrates, influencing RNA stability and decay. It is a key effector in post-transcriptional gene regulation, acting on precursor and mature miRNAs and participating in pathways that couple RNA tailing to exonucleolytic turnover. ZCCHC6 has been linked to regulation of innate immune and stress-responsive transcriptomes through modulation of RNA processing and surveillance. Dysregulated RNA uridylation and altered ZCCHC6 activity have been associated with perturbations in miRNA homeostasis and gene-expression programs relevant to cancer and inflammatory phenotypes.
ZCCHC6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZCCHC6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZCCHC6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZCCHC6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZCCHC6 protein expression.
This CRISPR knockout system enables efficient generation of ZCCHC6-deficient cell models for investigation of ZCCHC6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZCCHC6 exon(s) critical for ZCCHC6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZCCHC6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZCCHC6 CRISPR/Cas9 KO Plasmid (h) and ZCCHC6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZCCHC6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZCCHC6 HDR Plasmid (h) and ZCCHC6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZCCHC6 homology arms to support homology-directed repair at defined ZCCHC6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.