Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
WWOX Double Nickase Plasmid (h): sc-403070-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WWOX Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- WWOX Double Nickase Plasmid (h) and WWOX Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting WWOX. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WWOX Antibody (C-7): sc-374449
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WWOX Double Nickase Plasmid (h) | sc-403070-NIC | 20 µg | $410.00 | |||
WWOX Double Nickase Plasmid (h2) | sc-403070-NIC-2 | 20 µg | $410.00 |
WWOX (WW domain-containing oxidoreductase) is a tumor suppressor encoded within the common fragile site FRA16D and functions as a scaffolding protein that integrates signaling at focal adhesions, mitochondria, and the nucleus. Through its WW domains, WWOX binds proline-rich partners to modulate apoptosis, DNA damage responses, and cellular stress signaling, with reported connections to p53- and TGF-β–associated pathways and regulation of transcriptional programs. Loss or reduced expression of WWOX is frequently observed in multiple cancer types and is linked to genomic instability and altered cell survival. In the nervous system, WWOX dysfunction is associated with neurodevelopmental phenotypes, supporting its broad role in controlling proliferation–death balance and cellular homeostasis.
WWOX Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the WWOX locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within WWOX. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt WWOX function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of WWOX-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.