Anticorpo WT1 (H-1) raccomandato per il rilevamento di WT1 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo WT1 (H-1): sc-393498

Anticorpo WT1 (H-1) valutazione 3.3 di 5 di 12.
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Schede tecniche

    • WT1 Antibody H-1 è un monoclonale di topo IgM κ, citato in 5 pubblicazioni, fornito in 200 µg/ml
    • specifico per un epitopo mappato tra gli amminoacidi 123-164 nella regione interna a WT1 di origine human
    • raccomandato per il rilevamento di WT1 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA; anche reattivo con ulteriori specie, incluse e bovine and porcine
    • Contattaci per ricevere un FREE 10 µg sample di WT1 (H-1): sc-393498.
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I would like to use the WT-1 (H-1) antibody to stain for podocytes in kidney organoids. Do you have any secondary antibody to recommend for fluorescence microscopy?

Richiesta da: ValeM
Thank you for your question. Here is a link to our secondary antibodies: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Risposta da: Tech Service
Data di pubblicazione: 2019-05-21

Hi, can I ask for the antibody WT1 (H1) insert. Actually I'm looking for WT1 which detect carboxyl Terminus or C-Terminus. Is this is the correct one? to rule out DSRCT (Desmoplastic Small Round Cell Tumours). Can I have the sample for evaluation? TQ

Richiesta da: RUZA
Thank you for your question. This mouse monoclonal IgM antibody is specific for an epitope mapping between amino acids 123-164 within an internal region of WT1 of human origin. It does not specifically recognize the C-terminus. We do not currently offer any WT1 antibodies with C-terminal epitopes. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Risposta da: Technical Support
Data di pubblicazione: 2019-03-03

I am using WT1 (H-1): sc-393498 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Richiesta da: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6.0) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support 15
Data di pubblicazione: 2017-01-20
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Rated 1 di 5 di da 免疫組織化学でうまく機能しない 精巣のパラフィン切片を使って免疫蛍光染色を行いましたが、 セルトリ細胞の核が全く染色されませんでした。
Data di pubblicazione: 2020-12-23
Rated 5 di 5 di da Dr Sadek Ab work good with dilution 1:200 and 2 hrs incubation at 37ºc and overnight at 4 ºc
Data di pubblicazione: 2018-11-21
Rated 1 di 5 di da No IHC or IF staining at all. We acquire a sample and perform IHC and IF on 4um mouse kidney section with M.O.M. kit. None of them works. Podocytes does not have nuclear staining of WT-1, nor has cytoplasmic staining. It`s just completely negative. I really hope the santa Cruz can resume C-19 (sc-192) since it works perfectly on frozen sections with 4% PFA fixation of 20min.
Data di pubblicazione: 2018-08-23
Rated 1 di 5 di da WT1 antibody Sc-393498 Purchased this antibody recently. Tried it on WB and IP-MS experiments. Didn't work in either. :((
Data di pubblicazione: 2018-05-07
Rated 1 di 5 di da 染的都是背景 I applied a sample sack in Sep. and then I did IF in both testis and heart. Results showed that the nonspecific staining as it stained in cytoplasm and myocardiocytes where supposed not be appear.
Data di pubblicazione: 2017-10-12
Rated 5 di 5 di da Good, BUT Antibody is perfect, but it maybe unsuitable to replace C-19, because my aim is detect EWS-WT1. The service of SCBT is very nice!
Data di pubblicazione: 2017-07-31
Rated 4 di 5 di da 抗体使用评论 用这个抗体做WB实验,使用了NB4细胞系,加药处理测WT1变化。实验结果还行。但是还需要重复实验验证结果。
Data di pubblicazione: 2017-07-21
Rated 5 di 5 di da Cost-effective Our laboratory has been using your company's antibody, it is very practical!
Data di pubblicazione: 2017-07-05
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