Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Vasohibin-1 CRISPR/Cas9 KO Plasmid (h): sc-403526
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Vasohibin-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Vasohibin-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Vasohibin-1 Antibody (C-6): sc-365541
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Vasohibin-1 CRISPR/Cas9 KO Plasmid (h) | sc-403526 | 20 µg | $397.00 |
Overview
VASH1 encodes vasohibin-1, an endothelial-derived regulator of angiogenesis that is induced by pro-angiogenic cues and functions in negative-feedback control of new vessel formation. Vasohibin-1 has been linked to modulation of endothelial cell proliferation and migration, vascular remodeling, and microtubule dynamics through its tubulin carboxypeptidase activity and interactions with cofactors such as SVBP. Altered VASH1 expression has been reported in tumor-associated vasculature and in conditions involving pathological neovascularization and chronic inflammation, supporting its relevance to studies of vascular homeostasis. As a result, VASH1 is frequently examined in pathways governing endothelial stress responses, tissue ischemia, and extracellular signaling that shapes the angiogenic microenvironment.
Vasohibin-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VASH1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VASH1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VASH1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Vasohibin-1 protein expression.
This CRISPR knockout system enables efficient generation of VASH1-deficient cell models for investigation of Vasohibin-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VASH1 exon(s) critical for Vasohibin-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VASH1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Vasohibin-1 CRISPR/Cas9 KO Plasmid (h) and Vasohibin-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VASH1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Vasohibin-1 HDR Plasmid (h) and Vasohibin-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VASH1 homology arms to support homology-directed repair at defined VASH1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.