USP14 CRISPR/Cas9 KO Plasmid (m): sc-425567
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP14 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the USP14 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: USP14 Antibody (6E6): sc-100630
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP14 CRISPR/Cas9 KO Plasmid (m) | sc-425567 | 20 µg | $397.00 |
Overview
Usp14 encodes ubiquitin-specific peptidase 14 (USP14), a proteasome-associated deubiquitinating enzyme that trims ubiquitin chains and regulates substrate processing at the 26S proteasome. By modulating ubiquitin recycling and protein turnover, USP14 influences proteostasis, stress responses, and signaling outputs linked to ubiquitin-dependent degradation. In mouse cells, altered USP14 activity has been connected to disturbances in neuronal maintenance and synaptic function, consistent with the importance of balanced proteasome dynamics in neurobiology. USP14 also intersects with pathways governing protein quality control and cellular homeostasis, making it relevant for mechanistic studies of ubiquitin–proteasome system dysfunction.
USP14 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Usp14 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Usp14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Usp14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish USP14 protein expression.
This CRISPR knockout system enables efficient generation of Usp14-deficient cell models for investigation of USP14 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Usp14 exon(s) critical for USP14 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Usp14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by USP14 CRISPR/Cas9 KO Plasmid (m) and USP14 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Usp14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by USP14 HDR Plasmid (m) and USP14 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Usp14 homology arms to support homology-directed repair at defined Usp14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.