Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
USP10 Double Nickase Plasmid (h): sc-404281-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- USP10 Double Nickase Plasmid (h) and USP10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting USP10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: USP10 Antibody (C-7): sc-365828
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP10 Double Nickase Plasmid (h) | sc-404281-NIC | 20 µg | $410.00 | |||
USP10 Double Nickase Plasmid (h2) | sc-404281-NIC-2 | 20 µg | $410.00 |
USP10 (ubiquitin specific peptidase 10) encodes a deubiquitinating enzyme that removes ubiquitin chains from select substrates to regulate protein stability, trafficking, and stress responses. USP10 has been linked to ubiquitin-dependent control of DNA damage signaling and p53 pathway modulation through deubiquitination of regulatory proteins, influencing cell-cycle progression and apoptosis. It also interfaces with autophagy and endosomal dynamics, supporting proteostasis under cellular stress. Dysregulated USP10 activity and expression have been reported across multiple cancer and neurobiology contexts, making it relevant for mechanistic studies of ubiquitin signaling networks and genotype–phenotype relationships.
USP10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the USP10 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within USP10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt USP10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of USP10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.