UQCRC1 Double Nickase Plasmid (h): sc-404435-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UQCRC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UQCRC1 Double Nickase Plasmid (h) and UQCRC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UQCRC1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UQCRC1 Double Nickase Plasmid (h) | sc-404435-NIC | 20 µg | $410.00 | |||
UQCRC1 Double Nickase Plasmid (h2) | sc-404435-NIC-2 | 20 µg | $410.00 |
UQCRC1 encodes a core structural subunit of mitochondrial complex III (ubiquinol–cytochrome c reductase) in the inner membrane, supporting electron transfer within the respiratory chain and contributing to proton motive force generation for oxidative phosphorylation. By stabilizing assembly and function of complex III, UQCRC1 influences cellular ATP production, reactive oxygen species balance, and mitochondrial membrane potential. Perturbation of complex III components can disrupt metabolic homeostasis and stress signaling, linking UQCRC1 to pathways regulating bioenergetics, apoptosis susceptibility, and mitochondrial quality control. Altered mitochondrial respiration and complex III integrity are recurrent features in studies of neurodegeneration, cardiometabolic dysfunction, and cancer cell metabolic reprogramming, making UQCRC1 a useful target in mitochondrial biology research.
UQCRC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UQCRC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UQCRC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UQCRC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UQCRC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.