Anticorpo UDP-GlcDH (A-9) raccomandato per il rilevamento di UDP-GlcDH di origine mouse, rat e human in WB, IP, IF e ELISA

Anticorpo UDP-GlcDH (A-9): sc-393438

Anticorpo UDP-GlcDH (A-9) valutazione 5.0 di 5 di 2.
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Schede tecniche

    • UDP-GlcDH Antibody A-9 è un monoclonale di topo IgG1 fornito in 200 µg/ml
    • specifico per un epitopo mappato tra gli amminoacidi 459-486 al C-terminus di UDP-GlcDH di origine human
    • raccomandato per il rilevamento di UDP-GlcDH di origine mouse, rat e human in WB, IP, IF e ELISA
    • peptide bloccante, sc-393438 P
    • Vedi m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), le nostre alternative ricombinanti ai reagenti secondari convenzionali anti-mouse IgG altamente raccomandate.

What blotting conditions were used for the blot? dilution? blocking agent? I used the antibody at 1:1000 in 5% milk/TBST. It was almost clean enough but could be a little better.

Richiesta da: Joy N
Thank you for your question. You can find dilution recommendations for each antibody on their respective datasheets. Our protocol for western blotting that is used by our labs as well can be found under this link: https://www.scbt.com/resources/protocols/western-immuno-blotting We recommend UltraCruz® Blocking Reagent: sc-516214. Alternatively, a milk-based blocking buffer will produce a cleaner blot while BSA will provide stronger signals. Incubating the blot at a low dilution over night at 4 °C can also help to produce a robust and specific signal in many cases.
Risposta da: Tech Support Europe
Data di pubblicazione: 2020-07-09

What application is the blocking peptide sc-393438 P appropriate for?

Richiesta da: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Risposta da: Technical Support
Data di pubblicazione: 2017-02-28
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Rated 5 di 5 di da Produced beautiful immunofluorescence nuclear Produced beautiful immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Data di pubblicazione: 2015-02-23
Rated 5 di 5 di da Nice Western Blot data of UDP Nice Western Blot data of UDP-GlcDH expression in Hep G2, HeLa and A-431 whole cell lysates and human liver tissue extract . -SCBT QC
Data di pubblicazione: 2014-04-23
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