UBXN2B CRISPR/Cas9 KO Plasmid (h): sc-407871
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBXN2B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UBXN2B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBXN2B CRISPR/Cas9 KO Plasmid (h) | sc-407871 | 20 µg | $397.00 |
Overview
UBXN2B (UBX domain protein 2B) is a UBX family adaptor implicated in ubiquitin-dependent protein quality control by linking polyubiquitinated substrates to the AAA+ ATPase p97/VCP for extraction and downstream proteasomal processing. Through this role, UBXN2B contributes to regulation of ER-associated degradation (ERAD), turnover of misfolded or damaged proteins, and maintenance of proteostasis during cellular stress. Perturbation of p97–UBX cofactor networks is broadly relevant to pathways governing cell cycle progression, DNA damage responses, and stress signaling that shape cell viability and differentiation programs. As a node in ubiquitin–proteasome system (UPS) circuitry, UBXN2B is of interest for mechanistic studies in contexts where proteostasis imbalance and aberrant protein clearance contribute to disease-relevant phenotypes.
UBXN2B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UBXN2B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UBXN2B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UBXN2B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UBXN2B protein expression.
This CRISPR knockout system enables efficient generation of UBXN2B-deficient cell models for investigation of UBXN2B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UBXN2B exon(s) critical for UBXN2B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UBXN2B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UBXN2B CRISPR/Cas9 KO Plasmid (h) and UBXN2B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UBXN2B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UBXN2B HDR Plasmid (h) and UBXN2B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UBXN2B homology arms to support homology-directed repair at defined UBXN2B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.