TMEM132B CRISPR/Cas9 KO Plasmid (h): sc-414044
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMEM132B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TMEM132B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMEM132B CRISPR/Cas9 KO Plasmid (h) | sc-414044 | 20 µg | $397.00 |
Overview
TMEM132B encodes a single-pass transmembrane protein of the TMEM132 family that is enriched in the nervous system and associated with membrane organization and cell–cell/ECM interactions. Emerging studies implicate TMEM132 proteins in cytoskeletal coupling and adhesion-related processes that influence neurite outgrowth, synaptic connectivity, and cellular morphology. TMEM132B expression and genetic variation have been explored in neurodevelopmental and neuropsychiatric contexts, supporting its relevance for investigating pathways that shape neuronal network formation and maintenance. Functional interrogation of TMEM132B can help delineate how transmembrane scaffolding interfaces with signaling modules governing neuronal differentiation and stress-responsive remodeling.
TMEM132B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TMEM132B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TMEM132B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TMEM132B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TMEM132B protein expression.
This CRISPR knockout system enables efficient generation of TMEM132B-deficient cell models for investigation of TMEM132B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TMEM132B exon(s) critical for TMEM132B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TMEM132B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TMEM132B CRISPR/Cas9 KO Plasmid (h) and TMEM132B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TMEM132B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TMEM132B HDR Plasmid (h) and TMEM132B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TMEM132B homology arms to support homology-directed repair at defined TMEM132B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.