Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TGase2 Double Nickase Plasmid (m): sc-423375-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TGase2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TGase2 Double Nickase Plasmid (m) and TGase2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Tgm2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TGase2 Antibody (4G3): sc-73612
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TGase2 Double Nickase Plasmid (m) | sc-423375-NIC | 20 µg | $410.00 |
Mouse Tgm2 encodes transglutaminase 2 (TGase2), a multifunctional Ca²⁺-dependent enzyme that catalyzes protein crosslinking, deamidation, and polyamination reactions, thereby remodeling extracellular matrix and stabilizing protein assemblies. TGase2 also functions as a GTP-binding signaling protein and participates in integrin-mediated adhesion, cytoskeletal dynamics, and regulation of NF-κB- and TGF-β-associated stress and inflammatory responses. Through these activities, TGase2 influences apoptosis, autophagy, wound repair, and fibrotic remodeling, and altered TGase2 expression or activity has been linked to inflammatory disease models, tissue fibrosis, neurodegeneration, and tumor microenvironment biology. Tgm2 is therefore widely used as a node for studying matrix biology, immune signaling, and cellular stress adaptation in mouse systems.
TGase2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Tgm2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Tgm2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Tgm2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Tgm2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.