Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TGase2 CRISPR/Cas9 KO Plasmid (m): sc-423375
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TGase2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TGase2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TGase2 Antibody (4G3): sc-73612
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TGase2 CRISPR/Cas9 KO Plasmid (m) | sc-423375 | 20 µg | $397.00 |
Overview
Mouse Tgm2 encodes transglutaminase 2 (TGase2), a multifunctional Ca2+-dependent enzyme that catalyzes protein crosslinking, deamidation, and polyamination while also exhibiting GTP-binding/scaffold activities. TGase2 regulates extracellular matrix remodeling, cell adhesion and migration, apoptosis, and inflammatory signaling, interfacing with pathways such as NF-κB, TGF-β, integrin/FAK, and wound-healing programs. Its activity influences cytoskeletal dynamics and matrix stiffness, linking Tgm2 to fibrotic remodeling and tissue repair responses. Dysregulated TGase2 function has been associated with neuroinflammation, metabolic stress adaptation, and tumor microenvironment remodeling in preclinical models, motivating mechanistic studies in relevant mouse cell types.
TGase2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tgm2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tgm2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tgm2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TGase2 protein expression.
This CRISPR knockout system enables efficient generation of Tgm2-deficient cell models for investigation of TGase2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tgm2 exon(s) critical for TGase2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tgm2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TGase2 CRISPR/Cas9 KO Plasmid (m) and TGase2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tgm2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TGase2 HDR Plasmid (m) and TGase2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tgm2 homology arms to support homology-directed repair at defined Tgm2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.