TFEC CRISPR/Cas9 KO Plasmid (h): sc-406102
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TFEC CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TFEC genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TFEC Antibody (E-12): sc-515031
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFEC CRISPR/Cas9 KO Plasmid (h) | sc-406102 | 20 µg | $397.00 |
Overview
TFEC (transcription factor EC) encodes a basic helix–loop–helix leucine zipper transcription factor in the MiT/TFE family that binds E-box motifs to regulate lineage-restricted gene programs. It is most prominently studied in myeloid-lineage and pigment cell biology, where it can influence differentiation state and transcriptional networks that intersect with immune signaling and cellular stress responses. TFEC activity contributes to control of transcriptional outputs involved in cellular identity and adaptation to environmental cues. Dysregulated MiT/TFE-family transcriptional programs, including TFEC-associated networks, have been implicated in aberrant differentiation and cancer-related gene expression signatures, supporting mechanistic studies in oncogenic and immunobiology contexts.
TFEC CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TFEC gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TFEC together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TFEC open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TFEC protein expression.
This CRISPR knockout system enables efficient generation of TFEC-deficient cell models for investigation of TFEC signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TFEC exon(s) critical for TFEC function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TFEC genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TFEC CRISPR/Cas9 KO Plasmid (h) and TFEC CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TFEC locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TFEC HDR Plasmid (h) and TFEC HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TFEC homology arms to support homology-directed repair at defined TFEC target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.