TCP-11 CRISPR/Cas9 KO Plasmid (m): sc-423314
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TCP-11 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TCP-11 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TCP-11 CRISPR/Cas9 KO Plasmid (m) | sc-423314 | 20 µg | $397.00 |
Overview
Tcp11 encodes the sperm-associated protein TCP-11, a testis-enriched factor implicated in late spermatogenesis and the functional maturation of spermatozoa. TCP-11 has been linked to processes that shape flagellar structure and regulate motility, influencing capacitation-associated signaling and fertilization competence. In mouse models, altered Tcp11 expression or function is associated with reduced sperm quality and subfertility phenotypes, making it relevant for studying genetic contributions to male reproductive disorders. Its restricted expression profile also supports its use as a marker in germ cell differentiation and reproductive toxicology research.
TCP-11 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tcp11 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tcp11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tcp11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TCP-11 protein expression.
This CRISPR knockout system enables efficient generation of Tcp11-deficient cell models for investigation of TCP-11 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tcp11 exon(s) critical for TCP-11 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tcp11 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TCP-11 CRISPR/Cas9 KO Plasmid (m) and TCP-11 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tcp11 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TCP-11 HDR Plasmid (m) and TCP-11 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tcp11 homology arms to support homology-directed repair at defined Tcp11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.