TAZ Double Nickase Plasmid (h): sc-400320-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TAZ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TAZ Double Nickase Plasmid (h) and TAZ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting WWTR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TAZ Antibody (1F1): sc-293183
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAZ Double Nickase Plasmid (h) | sc-400320-NIC | 20 µg | $410.00 | |||
TAZ Double Nickase Plasmid (h2) | sc-400320-NIC-2 | 20 µg | $410.00 |
WWTR1 encodes the transcriptional co-activator TAZ (TAZ/WWTR1), a key downstream effector of the Hippo signaling pathway that integrates mechanical cues, cell polarity, and GPCR inputs to regulate gene expression programs controlling proliferation, survival, differentiation, and epithelial–mesenchymal plasticity. TAZ shuttles between cytoplasm and nucleus in response to LATS1/2-mediated phosphorylation, partnering with TEAD family transcription factors to modulate targets involved in tissue growth and extracellular matrix remodeling. Dysregulated TAZ activity has been linked to altered contact inhibition and aberrant stem-like transcriptional states, making WWTR1 a commonly interrogated node in studies of cancer biology, fibrosis-associated signaling, and organ development. Its crosstalk with Wnt/β-catenin, TGF-β/SMAD, and mechanotransduction pathways further supports its relevance for dissecting context-dependent transcriptional control.
TAZ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the WWTR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within WWTR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt WWTR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of WWTR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.