Synaptotagmin II CRISPR/Cas9 KO Plasmid (m): sc-423244
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Synaptotagmin II CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Synaptotagmin II genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Synaptotagmin II Antibody (26): sc-136089
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Synaptotagmin II CRISPR/Cas9 KO Plasmid (m) | sc-423244 | 20 µg | $397.00 |
Overview
Syt2 encodes synaptotagmin II, a Ca2+-binding membrane protein that functions as a key fast Ca2+ sensor for synaptic vesicle exocytosis and synchronous neurotransmitter release. Through its C2 domains, synaptotagmin II couples action potential–evoked Ca2+ influx to SNARE-dependent membrane fusion, shaping presynaptic release probability and short-term plasticity. In mouse neurons and neuromuscular junctions, Syt2 expression is closely linked to rapid transmission in defined circuits and motor pathways. Dysregulation of Ca2+-triggered vesicle fusion and presynaptic release mechanisms is broadly relevant to studies of neurophysiology and neurologic disease mechanisms where synaptic dysfunction is a central feature.
Synaptotagmin II CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Syt2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Syt2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Syt2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Synaptotagmin II protein expression.
This CRISPR knockout system enables efficient generation of Syt2-deficient cell models for investigation of Synaptotagmin II signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Syt2 exon(s) critical for Synaptotagmin II function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Syt2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Synaptotagmin II CRISPR/Cas9 KO Plasmid (m) and Synaptotagmin II CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Syt2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Synaptotagmin II HDR Plasmid (m) and Synaptotagmin II HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Syt2 homology arms to support homology-directed repair at defined Syt2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.