Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Stim1 Double Nickase Plasmid (h): sc-401311-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Stim1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Stim1 Double Nickase Plasmid (h) and Stim1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STIM1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Stim1 Antibody (A-8): sc-166840
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stim1 Double Nickase Plasmid (h) | sc-401311-NIC | 20 µg | $410.00 | |||
Stim1 Double Nickase Plasmid (h2) | sc-401311-NIC-2 | 20 µg | $410.00 |
STIM1 (stromal interaction molecule 1) is an ER-resident Ca2+ sensor that couples depletion of luminal calcium stores to activation of store-operated calcium entry (SOCE) by engaging ORAI channels at ER–plasma membrane junctions. Through regulation of cytosolic Ca2+ dynamics, Stim1 coordinates downstream signaling pathways including calcineurin–NFAT transcriptional programs, cytoskeletal remodeling, and calcium-dependent control of proliferation and secretion. STIM1-dependent SOCE is central to immune cell activation and broader stress-response signaling, and altered STIM1 function or expression has been linked to immunological dysfunction, myopathies, and tumor-associated calcium signaling phenotypes. These properties make STIM1 a widely used node for dissecting calcium signaling networks and stimulus-coupled transcriptional responses in human cell models.
Stim1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STIM1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STIM1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STIM1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STIM1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.