STC2 CRISPR/Cas9 KO Plasmid (m): sc-423183
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- STC2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the STC2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: STC2 Antibody (2B11): sc-293388
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
STC2 CRISPR/Cas9 KO Plasmid (m) | sc-423183 | 20 µg | $397.00 |
Overview
Stc2 encodes stanniocalcin 2 (STC2), a secreted glycoprotein implicated in calcium and phosphate homeostasis and cellular adaptation to metabolic and microenvironmental stress. In mouse cells, STC2 expression is regulated by stress-responsive signaling and is commonly linked to endoplasmic reticulum stress and unfolded protein response programs, supporting survival under hypoxia or nutrient limitation. STC2 has been connected to modulation of growth factor signaling, redox balance, and proteostasis, positioning it as a functional node in pathways that influence proliferation and differentiation. Altered STC2 activity has been reported across diverse disease-relevant contexts, including cancer-associated phenotypes, inflammatory stress, and metabolic dysregulation, making Stc2 a useful target for mechanistic studies in mammalian models.
STC2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Stc2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Stc2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Stc2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish STC2 protein expression.
This CRISPR knockout system enables efficient generation of Stc2-deficient cell models for investigation of STC2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Stc2 exon(s) critical for STC2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Stc2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by STC2 CRISPR/Cas9 KO Plasmid (m) and STC2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Stc2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by STC2 HDR Plasmid (m) and STC2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Stc2 homology arms to support homology-directed repair at defined Stc2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.