Stat2 Double Nickase Plasmid (h): sc-417454-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Stat2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Stat2 Double Nickase Plasmid (h) and Stat2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STAT2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Stat2 Antibody (B-3): sc-514193
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stat2 Double Nickase Plasmid (h) | sc-417454-NIC | 20 µg | $410.00 | |||
Stat2 Double Nickase Plasmid (h2) | sc-417454-NIC-2 | 20 µg | $410.00 |
STAT2 encodes Stat2, a central transcription factor in type I and type III interferon signaling that forms the ISGF3 complex with STAT1 and IRF9 to drive expression of interferon-stimulated genes. Following IFNAR engagement, JAK1 and TYK2 phosphorylate Stat2, enabling nuclear translocation and regulation of antiviral defense, antigen presentation, and innate immune crosstalk. Stat2 activity influences cytokine networks and cell-intrinsic restriction pathways, shaping responses to viral infection and inflammatory cues. Dysregulated STAT2 signaling has been associated with inborn errors of immunity and altered susceptibility to infectious and immune-mediated disease phenotypes, making it a frequent target in mechanistic immunology research.
Stat2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STAT2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STAT2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STAT2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STAT2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.