Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SSTR2 Double Nickase Plasmid (h): sc-401618-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SSTR2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SSTR2 Double Nickase Plasmid (h) and SSTR2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SSTR2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SSTR2 Antibody (A-8): sc-365502
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SSTR2 Double Nickase Plasmid (h) | sc-401618-NIC | 20 µg | $410.00 | |||
SSTR2 Double Nickase Plasmid (h2) | sc-401618-NIC-2 | 20 µg | $410.00 |
SSTR2 encodes somatostatin receptor 2 (SSTR2), a G protein–coupled receptor that binds somatostatin to regulate neuroendocrine secretion and cell signaling. Upon activation, SSTR2 typically couples to Gi/o proteins to reduce cAMP production, modulate calcium and potassium channel activity, and influence downstream pathways such as MAPK/ERK and PI3K signaling, thereby shaping proliferation, differentiation, and secretion programs. Receptor internalization and recycling further control signal duration and receptor availability at the plasma membrane. Altered SSTR2 expression or signaling has been studied in the context of neuroendocrine biology and tumor-associated receptor profiling, making it a useful target for mechanistic studies of GPCR regulation and endocrine signaling networks.
SSTR2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SSTR2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SSTR2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SSTR2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SSTR2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.