SREC CRISPR/Cas9 KO Plasmid (h): sc-406838
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SREC CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SREC genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SREC CRISPR/Cas9 KO Plasmid (h) | sc-406838 | 20 µg | $397.00 |
Overview
SCARF1 encodes scavenger receptor expressed by endothelial cells (SREC), a class F scavenger receptor that binds and internalizes a broad range of ligands, including modified lipoproteins and polyanionic macromolecules. SREC contributes to endocytic trafficking, cellular adhesion, and clearance of extracellular material, linking vascular endothelium and immune surveillance to homeostatic uptake pathways. Through its roles in ligand recognition and internalization, SCARF1 is studied in processes such as inflammation, oxidative stress responses, and vascular biology where altered scavenger receptor activity can influence tissue microenvironments. Dysregulation of scavenger receptor pathways has been associated with cardiometabolic and inflammatory disease mechanisms, supporting SCARF1 as a useful target for dissecting endothelial–immune interactions in human model systems.
SREC CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SCARF1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SCARF1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SCARF1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SREC protein expression.
This CRISPR knockout system enables efficient generation of SCARF1-deficient cell models for investigation of SREC signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SCARF1 exon(s) critical for SREC function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SCARF1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SREC CRISPR/Cas9 KO Plasmid (h) and SREC CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SCARF1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SREC HDR Plasmid (h) and SREC HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SCARF1 homology arms to support homology-directed repair at defined SCARF1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.