Sp1 CRISPR/Cas9 KO Plasmid (m): sc-423094
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sp1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sp1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sp1 Antibody (1C6): sc-420
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sp1 CRISPR/Cas9 KO Plasmid (m) | sc-423094 | 20 µg | $397.00 |
Overview
Sp1 (specificity protein 1) is a ubiquitously expressed zinc-finger transcription factor that binds GC-rich promoter elements to regulate basal and inducible transcription across diverse gene programs. In mouse cells, Sp1 integrates signaling inputs from MAPK/ERK, PI3K/AKT, and stress-responsive pathways to coordinate cell-cycle progression, DNA damage responses, chromatin remodeling, and metabolic homeostasis. Through interactions with co-regulators such as p300/CBP and HDAC complexes, Sp1 helps shape transcriptional output for genes involved in proliferation, apoptosis, and differentiation. Dysregulated Sp1 activity has been implicated in oncogenic transcriptional networks, inflammatory signaling, and neurodegeneration-associated gene expression changes, making it a key node for mechanistic studies of transcriptional control.
Sp1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sp1 protein expression.
This CRISPR knockout system enables efficient generation of Sp1-deficient cell models for investigation of Sp1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sp1 exon(s) critical for Sp1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sp1 CRISPR/Cas9 KO Plasmid (m) and Sp1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sp1 HDR Plasmid (m) and Sp1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sp1 homology arms to support homology-directed repair at defined Sp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.