Sox9 Lentiviral Activation Particles (h): sc-400143-LAC

Human SOX9 encodes the transcription factor Sox9, a high-mobility group (HMG) box DNA-binding protein that controls lineage specification and tissue patterning during development. Sox9 regulates chondrogenesis and skeletogenesis through transcriptional networks that intersect with TGF-β/BMP signaling and coordinate extracellular matrix gene programs such as COL2A1 and ACAN. In epithelial contexts, SOX9 contributes to stem/progenitor cell maintenance and differentiation by integrating Wnt/β-catenin and Notch-associated transcriptional states. Dysregulated SOX9 activity is implicated in developmental disorders affecting cartilage formation and sex determination, and it is frequently studied for its role in oncogenic transcriptional reprogramming and cellular plasticity.

Sox9 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient SOX9 upregulation across a broader range of human cell types.

Sox9 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the SOX9 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous Sox9 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native SOX9 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.