SNX3 Double Nickase Plasmid (h): sc-404028-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SNX3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SNX3 Double Nickase Plasmid (h) and SNX3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SNX3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SNX3 Antibody (G-7): sc-376667
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNX3 Double Nickase Plasmid (h) | sc-404028-NIC | 20 µg | $410.00 | |||
SNX3 Double Nickase Plasmid (h2) | sc-404028-NIC-2 | 20 µg | $410.00 |
SNX3 (sorting nexin 3) is a PX-domain sorting nexin that binds phosphatidylinositol 3-phosphate on early endosomes and helps organize cargo sorting through the endosomal system. It is a key component of retromer-associated trafficking, supporting endosome-to-Golgi retrieval and regulated recycling of membrane proteins, thereby shaping receptor availability and downstream signaling. Through its roles in membrane remodeling and cargo selection, SNX3 influences processes such as nutrient uptake, signal attenuation, and maintenance of cellular polarity. Dysregulated endosomal trafficking pathways involving SNX3 and retromer function are frequently examined in the context of neurodegeneration, pathogen entry, and altered receptor signaling observed across multiple disease models.
SNX3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SNX3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SNX3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SNX3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SNX3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.